In vitro Antioxidant Activity of Various Extracts of whole Plant of Mucuna pruriens (Linn)

 

D.Satheesh kumar, A.Kottai Muthu*, A.Anton Smith and R.Manavalan

Abstract

The study was designed to examine the invitro antioxidant activities of various extracts of whole plant of Mucuna pruriens. The antioxidant activity was evaluated by DPPH (,-diphenyl-β-picrylhydrazyl) radical scavenging activity, Super Oxide Anion Scavenging Activity and Iron chelating activity with reference standard Rutin, Quercetin and EDTA respectively. The ethyl acetate extract of Mucuna pruriens was found to more effective in the DPPH radical scavenging activity. The IC50 of the ethyl acetate extract of Mucuna pruriens and Rutin were found to be 420μg/ml and 480μg/ml respectively. An IC50 value was found that ethyl acetate extract of Mucuna pruriens is more effective in scavenging superoxide radical than that of methanol and petroleum ether extract. But when compare to the all the three extracts with Quercetin (standard), the ethyl acetate extract of the Mucuna pruriens showed the similar result. The Iron chelating activity of the methanolic extract of Mucuna pruriens was found to most effective than that of petroleum ether & ethyl acetate extract. It is concluded that a whole plant of ethyl acetate and methanolic extract of Mucuna pruriens, which contains large amounts of phenolic compounds, exhibits high antioxidant and free radical scavenging activities. These in vitro assays indicate that this plant extracts is a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses